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Ranking of eight mAb1 CHO cell clones based on average cell specific productivity values for fed-batch and semi-perfusion operation with total and partial medium exchanges in CD CHO and HIP medium.

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Cell clone selection—impact of operation modes and medium exchange strategies on clone ranking

doi: 10.3389/fbioe.2024.1479633

Figure Lengend Snippet: Ranking of eight mAb1 CHO cell clones based on average cell specific productivity values for fed-batch and semi-perfusion operation with total and partial medium exchanges in CD CHO and HIP medium.

Article Snippet: Cells were cultivated in two commercially available media: (1) fed-batch specific CD CHO medium (Gibco ® , Thermo Fisher Scientific, Massachusetts, United States) and (2) perfusion-specific High Intensity Perfusion (HIP) medium (Gibco ® , Thermo Fisher Scientific, Massachusetts, United States), both supplemented with 4% 50X HT supplement (Gibco ® , Thermo Fisher Scientific, Massachusetts, United States).

Techniques: Clone Assay